Διερεύνηση των βημάτων του Πρωτοκόλλου της In Vitro Δοκιμής Συμβατότητας Νανοσωματιδίων σε Ερυθροκύτταρα (Bachelor thesis)

Αξιοπολύτου, Στυλιανή


When a research procedure is carried out in which biomaterials and parts of the organism take part, the main question that arises is whether these materials are compatible with the organism. Nanoparticles are materials which in recent years have dominated the medical community, due to their properties and their targeted action, which is why they are used in many applications. By extension, the investigation of the compatibility of nanomaterials is crucial for the course of the experiment and for the license of their use in living organisms, because, in some situations they may greatly worsen the condition of a patient or even lead to conditions incompatible with life. The purpose of this study was to to set up a protocol using standard techniques to overcome nanoparticles aggregation and to learn the interactions of Nanoakermanite with normal red blood cells after submitting the material to some procedures such as bath sonication and probe sonication in order to develop a hemocompatibility protocol. Also, we wanted to understand the action of Nanoakermanite on red blood cells and the effect of ultrasound on the material, and to create a Protocol that will contribute to the safer use of nanoparticles. The results showed that Nanoakermanite is not compatible with red blood cells at high concentrations, while it is at the lowest. In the bath sonication it was found that at 20 minutes in the majority of the experiments there was maximum hemolysis, while the temperature makes our material even more hemolytic. Lastly, probe sonication doesn’t play a special role in hemolysis compared to the material that had not undergone the procedure.
Institution and School/Department of submitter: Σχολή Επιστημών Υγείας - Τμήμα Βιοϊατρικών Επιστημών
Subject classification: Νανοτεχνολογία -- Έρευνα
Ερυθροκύτταρα
Νανοσωματίδια
Δοκιμή συμβατότητας (Αιματολογία)
Nanotechnology -- Research
Erythrocytes
Nanoparticles
Compatibility testing (Hematology)
Keywords: Νανοτεχνολογία;Νανοσωματίδια;Αιματοσυμβατότητα;In Vitro Δοκιμή Συμβατότητας;Nanotechnology;Nanoparticles;Blood compatibility;In Vitro compatibility test
Description: Πτυχιακή εργασία - Σχολή Επιστημών Υγείας - Τμήμα Βιοϊατρικών Επιστημών, 2023 (α/α 13962)
URI: http://195.251.240.227/jspui/handle/123456789/16743
Item type: bachelorThesis
General Description / Additional Comments: Πτυχιακή εργασία
Subject classification: Νανοτεχνολογία -- Έρευνα
Ερυθροκύτταρα
Νανοσωματίδια
Δοκιμή συμβατότητας (Αιματολογία)
Nanotechnology -- Research
Erythrocytes
Nanoparticles
Compatibility testing (Hematology)
Submission Date: 2024-07-13T19:05:58Z
Item language: el
Item access scheme: free
Institution and School/Department of submitter: Σχολή Επιστημών Υγείας - Τμήμα Βιοϊατρικών Επιστημών
Publication date: 2023-02-24
Bibliographic citation: Αξυπολίτου, Σ. (2023). Διερεύνηση των Βημάτων του Πρωτοκόλλου της In Vitro Δοκιμής Συμβατότητας Νανοσωματιδίων σε Ερυθροκύτταρα (Πτυχιακή εργασία). ΔΙ.ΠΑ.Ε.
Abstract: When a research procedure is carried out in which biomaterials and parts of the organism take part, the main question that arises is whether these materials are compatible with the organism. Nanoparticles are materials which in recent years have dominated the medical community, due to their properties and their targeted action, which is why they are used in many applications. By extension, the investigation of the compatibility of nanomaterials is crucial for the course of the experiment and for the license of their use in living organisms, because, in some situations they may greatly worsen the condition of a patient or even lead to conditions incompatible with life. The purpose of this study was to to set up a protocol using standard techniques to overcome nanoparticles aggregation and to learn the interactions of Nanoakermanite with normal red blood cells after submitting the material to some procedures such as bath sonication and probe sonication in order to develop a hemocompatibility protocol. Also, we wanted to understand the action of Nanoakermanite on red blood cells and the effect of ultrasound on the material, and to create a Protocol that will contribute to the safer use of nanoparticles. The results showed that Nanoakermanite is not compatible with red blood cells at high concentrations, while it is at the lowest. In the bath sonication it was found that at 20 minutes in the majority of the experiments there was maximum hemolysis, while the temperature makes our material even more hemolytic. Lastly, probe sonication doesn’t play a special role in hemolysis compared to the material that had not undergone the procedure.
Advisor name: Παπαλιάγκας, Βασίλειος
Examining committee: Παπαλιάγκας, Βασίλειος
Τσαμεσίδης, Ιωάννης
Publishing department/division: Σχολή Επιστημών Υγείας - Τμήμα Βιοϊατρικών Επιστημών
Publishing institution: ihu
Number of pages: 66
Appears in Collections:Πτυχιακές Εργασίες

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