Eureka! Institutional Repository

When a research procedure is carried out in which biomaterials and parts of the organism take part, the main question that arises is whether these materials are compatible with the organism. Nanoparticles are materials which in recent years have dominated the medical community, due to their properties and their targeted action, which is why they are used in many applications. By extension, the investigation of the compatibility of nanomaterials is crucial for the course of the experiment and for the license of their use in living organisms, because, in some situations they may greatly worsen the condition of a patient or even lead to conditions incompatible with life. The purpose of this study was to to set up a protocol using standard techniques to overcome nanoparticles aggregation and to learn the interactions of Nanoakermanite with normal red blood cells after submitting the material to some procedures such as bath sonication and probe sonication in order to develop a hemocompatibility protocol. Also, we wanted to understand the action of Nanoakermanite on red blood cells and the effect of ultrasound on the material, and to create a Protocol that will contribute to the safer use of nanoparticles. The results showed that Nanoakermanite is not compatible with red blood cells at high concentrations, while it is at the lowest. In the bath sonication it was found that at 20 minutes in the majority of the experiments there was maximum hemolysis, while the temperature makes our material even more hemolytic. Lastly, probe sonication doesn’t play a special role in hemolysis compared to the material that had not undergone the procedure.