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Brucellosis is considered a serious zoonotic disease that primarily affects sheep, goats and cattle. It is caused by bacteria of the genus Brucella. It is characterized by abortions in females initially and by genital infestation in males and infertility in both sexes secondary. The disease is being spread globally and is still considered endemic in the countries of the Mediterranean Basin and Greece. The significance of the disease for our country is great, not only because of the fact that is a danger to public health, but because it also leads to considerable economic losses to domestic livestock. In order to control the disease, brucellosis control and eradication programs have been carried out for decades, without however being possible to eradicate it. Man can be contaminated by consuming contaminated milk or cheese, which have not gone through the processes of pasteurization and ripening previously. In the present study, the endemicity of small ruminant brucellosis in the prefecture of Chania for the year 2014 is being studied. For the epidemiological study, the data of the Department of Agricultural Economics and Veterinary Medicine of Chania is being studied, which resulted from the serological tests that carried out during the 2014, when a total of 141 sheep and goats farming were examined. The data were collected by the Chania General Hospital and were exploited in order to investigate any human cases. The results of the study showed that the disease is still endemic in the county, as in the year 2014 there were positives for sheep and goat disease, but there was no recorded human case for the year 2014, when the epidemiological study was carried out.