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The present survey aims at investigating job satisfaction and burnout among Physical Education teachers working at primary schools in the geographical region of Epirus. Job burnout was evaluated according to Maslach’s model, which consists of three dimensions, namely emotional exhaustion, depersonalization and loss of personal accomplishment. 108 Physical Education teachers from all four districts of Epirus participated in the survey. A closed-ended questionnaire, consisting of four parts, was used for data collection. The first part of the questionnaire was concerned with demographics. The second part examined job satisfaction, based on the ESI (Employee Satisfaction Inventory), while the third part evaluated job burnout and was based on the MBI (Maslach Burnout Inventory). Finally, the fourth part dealt with role ambiguity and role conflict, two very important factors, closely related to job satisfaction and burnout. Results showed a negative correlation between job satisfaction and job burnout. It was found that P.E. teachers of Epirus are fairly satisfied by the nature of their profession. Moderate satisfaction was observed in relation to working conditions, facilities and the organization as a whole, whereas low satisfaction was observed in areas such as teachers’ salary, career prospects and their relation to the manager of the institution. These factors have a negative impact and may lead P.E. teachers to emotional exhaustion. A high degree of emotional exhaustion was generally observed. Concerning the three dimensions of emotional exhaustion suggested by Maslach, emotional exhaustion, depersonalization and personal accomplishment appeared to be at high, low and medium levels accordingly. Emotional exhaustion and low accomplishment are considered core factors in the emergence of job burnout. Additionally, demographics were found to differentiate the degree of job burnout to some extent. Furthermore, role ambiguity and role conflict were proven to play a major part in the emergence of burnout. High levels of role ambiguity and medium to low levels of role conflict were observed in our results.