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Background: Cancer is a challenging condition that affects not only adults but also children, and it can significantly impact their quality of life. Physical therapy has been recognized as a complementary approach in the management of children with cancer, but its effectiveness remains inconclusive. This study aimed to assess the effectiveness of physical therapy on the quality of life in children with cancer using modified questionnaires. Methods: Two questionnaires, the Pediatric Quality of Life Inventory (PedsQL) and the Minneapolis-Manchester Quality of Life (MMQL), were modified by adding specific physiotherapy-based questions related to physical function, mobility, pain management and level of physiotherapy services. The modified questionnaires were administered to 12 adult parents of children aged 2-8 years old with cancer and 13 children 8-17 years old with cancer. Demographic information was collected, and data were analyzed using descriptive statistics and appropriate statistical methods. Results: The findings from the modified questionnaires were analyzed, and the results were mixed. Some participants reported significant improvements in quality of life outcomes related to physical function and overall well-being, while others showed no significant effects. However, due to the small sample size and potential measurement bias introduced by the use of modified questionnaires, the findings should be interpreted with caution. Conclusions: The effectiveness of physical therapy in improving the quality of life in children with cancer remains inconclusive based on the results of this study. Further larger-scale studies with rigorous methodologies are warranted to confirm the findings and provide more robust evidence in this area. Physical therapy should be considered as part of a multimodal approach in the comprehensive care of children with cancer, with close collaboration between the oncology and physiotherapy teams to provide tailored and evidence-based care.