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The primary aim of the present study is to investigate the agronomic behaviour of soft wheat and its yield. Moreover, the quality of the crop grain at different nitrogen fertilization levels is examined along with different mechanisms of action. Additionally, the main objective of the study is to analyse modern fertilizers in order to compare them with the traditional ways of fertilization practice currently applied in Greece. This is achieved through the cultivation and measurement of the efficiency of "Acheloos" soft wheat, in terms of Nitrogen (N) usage in different forms, during the period 2016-17, at the experimental field of the Institute of Genetic Improvement and Phytogenetic Resources. Statistical analysis was pursued on a fully randomized experimental sample using IBM SPSS Statistics 24 software (SPSS Inc., Chicago. IL, USA) and the mean scores were compared to the criterion of the minimum significant difference. Regarding the number of plants per hectare, results did not support statistically significant differences. However, the findings concerning the height of the plant showed that in conventional fertilization the height was higher compared to the fertilizer-free process where the height was lower. As far as yield is concerned, the application of 9 lubricating units CRFN proved to give better results. The harvest index gave better results at the fertilization of 6.3 and 9 l.u. CRF N, while worse results were provided by the 0-fertilization condition. Moreover, the N concentration in the seed was lower at the null fertilization; the biomass had less N concentration at 0 and 9 l.u. CRF, whereas in conventional fertilization the N soil concentration was lower after the harvest. Additionally, the present study examines the concentration of phosphorus (P), potassium (K), zinc (Mn) and manganese (Mn) while results regarding protein, gluten, hectolitre weight and the weight of one thousand grains are also analysed. Finally, analysed that nitrogen fertilization increases photosynthetic efficiency and photosynthetic rate, while decreasing the photosynthetic efficacy of the varieties results from the decrease of soil moisture