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An effective dietetic service for maternity care is important for a number of reasons. A poor quality diet during pregnancy is associated with unhealthy maternal weight gain, preeclampsia, anemia, preterm birth or miscarriage. It is also associated with poor infant outcomes, including inadequate development, low birth weight, preterm birth, macrosomia and an increased risk of chronic diseases later in life. The American Dietetic Association recommends a safe, healthy and balanced diet and highlights the key nutrients folate, iron, calcium and vitamin D as particularly important considerations during pregnancy (American Dietetic Association, 2008). Despite the existence of such guidelines, the extent to which healthcare professionals adhere to them is currently unknown, at least in Greece. Purpose : This research aims to identify the level of nutritional knowledge that Greek pregnant women possess, related to the needs of a pregnancy and breastfeeding. We also focused on examining of their sources of information and whether the information provided to them is sufficient in order to form their nutritional habits so they can adequately meet the needs of themselves and their fetus. Methodology : Data collection was performed in the department of ultrasounds of the Gynecology and Obstetrics Clinic REA in Athens in May 2016. 154 pregnant women took part in the research during singleton pregnancies and regardless the trimester. To collect the data for this survey we used questionnaires. Results : Our sample showed that the majority of pregnant women in Greece holds moderate knowledge level of 52.6%, followed by women with good level of 42.9% and only 4.5% of pregnant women has been found to possess low nutritional knowledge on issues related to pregnancy and breastfeeding. By examining the correlations we found that the level of nutritional knowledge is not affected by the trimester of pregnancy, BMI, nor on the existence of pre-existing pregnancy. Nevertheless, it seems to be influenced by the level of education, since 66.6% of women with a good level of nutritional knowledge, also possessed high level of education (51.5% bachelor, 13.6% MA, 1.5% doctoral). Conclusions : We believe that there is room for improvement regarding the nutritional information that pregnant women in Greece receive. Probably, the level of nutritional knowledge of pregnant women would be improved if the advice on diet was individualized, given more clearly, offered multiple times during pregnancy and provided by health professionals specialized in nutrition .