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The purpose of this paper is to evaluate the factors that affect the preferences on alternative means of transportation, as well as the variables that determine the behavior of the passengers. The survey focuses on passengers who use the means of transportation in Thessaloniki. At this stage, the passengers are served by the Thessaloniki Urban Transport Organization, which is the only mean of mass transportation in the city, while the Metro project is under construction and the electric scooters have made their appearance in Thessaloniki. The addition of new means of transport to the city of Thessaloniki cause the interest for further research. Therefore, the determinants of passenger choices for the use of private vehicles, urban buses, metro and electric scooters were assessed. Also, inhibitory factors and incentives for the choice of different modes of transport were explored. To conduct the survey, the data were collected by random sampling using questionnaires. A total of 104 people participated in the survey, and a statistical analysis was performed with the help of the statistical program IBM SPSS (Superior Performance Software System) Statistics Version 23, in order to identify the factors that affect the behavior of the passengers. Finally, after analyzing and commenting on the results, proposals are made to increase the use of friendly means of transport, meaning the use of Public Transportation and Micro-Mobility.